Light-dark box test is traditionally used to assess the levels of anxiety, both in rodents and in Danio rerio (zebrafish). This test utilizes the scototaxis, an aversion to bright areas and preference for the dark, which is natural for zebrafish. Light-dark box test does not require pre-training.

 

Materials

Walls and dividers are made of matte foamed PVC (black and white), translucent wall connectors of acrylic. The arena is waterproof.

Dimensions

height	150 mm
width	100 mm
overall length	450 mm
length of white or dark chamber (till the insertable divider)	195 mm
length of the starting chamber (between the insertable dividers)	50 mm